Name: Rishabh Shaileshkumar Kalra
Lab Partners: Harshal
Date(s) of analysis: September 12th and September 19th.

Laboratory 1Environmental Testing
Results
a) Nutrient Agar Plate
Location of plate when exposed to the air: CCT-65 , A 206
Length of time exposed to the air: 2:50 pm to 3:50 pm
Describe the microbial growth on your plate. Include shape, color and relative abundance of growth.

There were 5 colonies of microbes seen.

Color- out of those five, 4 of them were white colored while 1 of them was black colored. Black microbial colony was seen on top of one of the white colonies.
Shape- colonies were irregularly round or oval shape separated by blank space
Growth- White colonies were found in the largest amount covering almost 80% of agar plate.

b) Agar Slant 1.
Location of Swabbing: On working platform, CCT-65, A206
Describe any microbial growth on your agar slant. Include shape, color and relative abundance of growth.

No microbial colonies found.
Microscopic study: No microbial organism found.

c) Agar Slant 2.
Location of Swabbing: Over the locker number #248
Describe any microbial growth on your agar slant. Include shape, color and relative abundance of growth.

No microbial colonies found.

Microscopic study: No microbial organism found
Lab 1 Questions
Why might some of the microorganisms in the environment be unable to grow on your nutrient agar plates?
Answer 1) there are various reasons for microorganisms to not grow on nutrient agar plates. They are as follows.

The plate has been contaminated with other microbes.

Due to insufficient nutrients available.

Some cultures do not show growth if the source culture is made of dead microbes.

Specific conditions should be maintained for specific microbial growth such as temp.

What are two factors that could change the effectiveness of soap or a disinfectant against microorganisms?
Answer 2) the 2 factors that could change the effectiveness are
Resistance from microorganisms.

Tolerance from a particular chemical or expired soap or disinfectant.

What is meant by the term “aseptic technique” and why do we practice it in the Microbiology Laboratory?
Answer 3) aseptic technique is a set of rules followed under controlled conditions to minimize the risk of contamination from pathogens. We practice it in the laboratory to produce accurate results and prevent microorganisms from entering and harming human body.

Reference(s)
No references used.